2024 Rpl22-ha小鼠

Rpl22-ha小鼠

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August undefined, 2024

WebDec 23, 2024 · Sox10 iCreERT2 /Rpl22 HA/+ animals received intraperitoneal injections of 100 µl Tamoxifen [MP Biomedicals,] dissolved in 10% ethanol, 90% sterile corn oil (final concentration 10 mg ml −1) for three days. Animals rested at least for one week after the final injection before any experiments were performed. Web011029 B6N.129- Rpl22 tm1.1Psam /J The RiboTag targeted mutation of the ribosomal protein L22 locus allows Cre-mediated hemagglutinin epitope tagging of ribosomes from …

Rpl22 is required for IME1 mRNA translation and meiotic induction in …

WebJan 10, 2024 · For Rpl22 and Like1 to directly regulate smad2 pre-mRNA splicing during gastrulation, Rpl22 and Like1 would have to be located in the nucleus. Indeed, immunofluorescence analysis of ectopically expressed HA-tagged RP revealed that Like1 and Rpl22 were located primarily in the nucleus at 6 and 10 hpf (Figures 4 A and S4 A). WebMechanistically, Rpl22 regulates Rpl22l1 directly by binding to an internal hairpin structure and repressing its expression. We propose that ribosome specificity may exist in … lawn tractors for sale in morris county nj

RPL22L1 - Wikipedia

Web2015). RiboTag mice carry a ribosomal protein Rpl22 allele with a floxed wild-type C-terminal exon followed by an identical C-terminal exon that has three HA epitopes inserted before the stop codon. Notably, Rpl22-HA is expressed under the control of its own promoter. Thus, in WebJun 5, 2024 · RPL22-HA and RPL22-V5 were only detected in IP fractions using HA or V5 antibodies, respectively, regardless of the order in which the IPs were performed. Thus, sequential IP can be used to separate epitope-tagged RPL22 from distinct cell populations. 3.3. Transcriptome-Wide Cell Type-Specific Enrichment in Mixed Species Co-Cultures … WebÐÏ à¡± á ÀF> þÿ { þÿÿÿ~ € ‚ ƒ ... lawn tractors for sale ebay

Rpl22-ha小鼠

sncRiboTag-Seq: Cell-type-specific RiboTag-Seq for cells in low ...

WebMar 19, 2024 · Crossing RiboTag mice (RPL22 HA/WT) with specific Cre-driver mice: Cre-lox recombination approach is commonly used as a tool for cell-type-specific gene expression. The RiboTag mice express HA-tagged ribosome protein RPL22 in Cre-expressing cells. Although this strategy is straightforward and efficient, there are several considerations in ... WebMar 29, 2024 · This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to …

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WebRibosomal protein L22 like 1 (RPL22L1), or eL22L1, is a protein that in humans is encoded by the RPL22L1 gene.. Function. eL22L1 is a ribosomal protein paralog of the eL22 protein of … WebMar 26, 2012 · 对表达Rpl22 HA 蛋白质的纯合子小鼠的大脑匀浆进行蔗糖密度梯度分析，然后再进行 Western blotting分析。图1F 蔗糖密度梯度为15%-50%，测核糖体总蛋白 …

WebNational Center for Biotechnology Information WebJun 18, 2024 · The Rpl22-HA gene can also be introduced into the animal by stereotaxic injection of an AAV-DIO-Rpl22-HA that is then activated in Cre-expressing cells. Both methods for tagging ribosomes facilitate the immunoprecipitation of ribosome-bound mRNAs and their analysis by qRT-PCR or RNA-Seq. This protocol will discuss the …

WebNational Center for Biotechnology Information WebFeb 24, 2024 · Rad21 with NexCre-dependent expression of an epitope-tagged ribosomal subunit (Rpl22-HA RiboTag; Ref. 39). We verified that NexCre-induced RPL22-HA expression was restricted to RAD21-depleted neurons (Supplementary Fig. 1a) and performed high throughput sequencing of Rpl22-HA RiboTag-associated mRNA (RiboTag RNA-seq). …

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WebAug 18, 2009 · Rpl22 ha-expressing homozygous mice, which express only the RPL22 ha protein, appear normal and are fertile. RPL22 ha Protein Is Incorporated into … lawn tractors for sale near harbor beach miWebAug 8, 2009 · Rpl22 ha-expressing homozygous mice, which express only the RPL22 ha protein, appear normal and are fertile. RPL22 ha Protein Is Incorporated into … lawn tractors for sale in bcWebJul 29, 2016 · In the absence of Rpl22 function, IME1 mRNA synthesis is normal in cultures induced to enter meiosis. ... Proteins were separated by 10 % SDS-PAGE, transferred to PVDF membranes and blots were probed with anti-HA (12CA5, Roche), anti-Tub1p (Developmental Studies Hybridoma Bank, University of Iowa), poly-clonal anti-Ume6 or anti-Pgk1p ... lawn tractors for sale in stockWebMay 7, 2024 · 为了证明Pten在内皮组织中被特异性敲除，研究人员采用了Ribotag小鼠模型，该小鼠模型的Rpl22（核糖体蛋白）基因序列上插入了loxp位点和HA位点，当Ribotag … kansas forestry service trees for sale kansas fsa office locatorWebOct 3, 2024 · Rpl22L1 is highly expressed in colon adenocarcinoma and correlates with poor prognosis. To further explore the extent of RPL22L1 induction in human colon cancer … lawn tractors for sale nbWebRPL22-HA and RPL22-V5 were only detected in IP fractions using HA or V5 antibodies, respectively, regardless of the order in which the IPs were performed. Thus, sequential IP can be used to separate epitope-tagged RPL22 from distinct cell populations. 3.3. Transcriptome-Wide Cell Type-Specific Enrichment in Mixed Species Co-Cultures Using … lawn tractors for sale alberta