WebJan 1, 2024 · For RPLC-MS and HILIC-MS the mass spectrometer was operated in the ESI+ mode with a capillary voltage of 1.5 kV, desolvation temperature of 550 °C and a cone voltage 120 V for HILIC-MS and 70 V for RPLC-MS. Full scan acquisition was performed with IDC on and a mass range of 400–7000 m/z with a scan time of 2 Hz. WebHowever, a recent study has evaluated the advantages and limitations of RPLC × HILIC compared to HILIC × RPLC for the analysis of phenolic compounds in food samples (Muller & de Villiers, 2024). The results showed that, although HILIC × RPLC separation was preferred from a chromatographic point of view (better resolution and peak capacity ...
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WebHILIC-RPLC (or vice versa) is the most orthogonal combination of liquid separation techniques when orthogonality is defined as the coverage fraction of a 2D separation space. Other techniques such as IC and SEC (Size Exclusion Chromatography) results in more peak grouping or poorer resolution in comparison to HILIC. WebMar 16, 2024 · HILIC is a chromatographic mode that is gaining in importance for the last few years, particularly with the growing need for analyzing a large variety of biologically active substances including pharmaceutical compounds, amino acids, peptides, neurotransmitters, oligosaccharides, carbohydrates, nucleotides, or nucleosides.1HILIC is … clean reserve reserve - skin
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WebHILIC Troubleshooting ‹ This section is not an exhaustive guide to HPLC column and instrument troubleshooting but an aid to help you understand and correct some of the more common chromatographic problems that could arise during HILIC separations. Common problems, possible causes, and possible solutions Insufficient analyte retention HILIC-RPLC (or vice versa) is the most orthogonal combination of liquid separation techniques when orthogonality is defined as the coverage fraction of a 2D separation space. Other techniques such as IC and SEC (Size Exclusion Chromatography) results in more peak grouping or poorer resolution in comparison to HILIC. WebHILIC uses the same mobile phases and systems as the more common reversed-phase liquid chromatography (RPLC). However, it employs a polar stationary phase to separate … clean reserve white fig \u0026 bourbon